A Procedure of Silver Staining for Nucleic Acids in Agarose Gels without Pretreatment or Drying Steps

✍ Scribed by Claudio C. Prieto; Raúl I. Leonardelli; Fabián E. Zalazar

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 326 KB
• Volume: 252
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1997.2288

✦ Synopsis

Methods

In this paper, we report a fast, simple, and reproduc-Agarose gel electrophoresis of DNA. Samples of Hinible staining protocol for nucleic acids in agarose gels dIII digests of l DNA and/or plasmidic DNA were run with a sensitivity in the order of 10 pg/mm 2 . It took on a horizontal 0.8% agarose/11 TAE gel (9 1 10 1 only three steps: fixation, incubation with silver ions, 0.4 cm) prepared from molecular biology grade agarose and development of the gels (total time 50 min). The (Sigma Chemical Co., St. Louis, MO). Electrophoresis resulting calibration curves (area vs ng of loaded DNA)

was performed at 7.5 V/cm for 2.5 h (10).

after a densitometric scanning of agarose gels stained with this procedure were linear up to 50 ng of double-