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A simple method for measuring protein degradation in bacteria

โœ Scribed by George W. Chang; Karen Fenton

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
318 KB
Volume
59
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

We describe a rapid and simple method for measuring protein degradation in bacteria. After cells have incorporated [l-"Clleucine, they are washed and resuspended in a medium containing unlabelled leucine. After an incubation period, the cell suspensions are treated with chloramine-T to decarboxylate the [Vlleucine released in the course of protein degradation. The "COz is trapped and counted.

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