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A simple spectrophotometric method for measurement of nematode populations

✍ Scribed by Thakor R. Patel; Bruce A. McFadden

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
348 KB
Volume
70
Category
Article
ISSN
0003-2697

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✦ Synopsis

An ordinary spectrophotometer was used to study growth rates and increases in population size of nematodes by optical density measurements of nematodes suspended in 30-40% sucrose (w/v) solutions. The sucrose solution retarded the movement of nematodes in suspension and thereby decreased the fluctuations normally observed in optical density. This method was effectively used to study growth rates and increases in population numbers of a free-living nematode, Caenorhabditis elegans. This procedure was also used to quantitate Ascaris lumbricoides eggs in a given sample. The limitations of this method when employed for establishing total nematode counts in a growing culture by direct spectrophotometric readings are discussed.

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