A simple method for trapping and measuring expired14CO2
β Scribed by Roger A. Davis; J. Philip Showalter; Fred Kern Jr.
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 101 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A method for estimation of 14CO2 present in blood and tissular samples is described. It is basically based on the introduction of large amounts of a gas mixture (95% O2, 5% CO2) in the samples which serves to remove the CO2 label by gas dilution. The gas phase is later captured in scintillation vial
We describe a rapid and simple method for measuring protein degradation in bacteria. After cells have incorporated [l-"Clleucine, they are washed and resuspended in a medium containing unlabelled leucine. After an incubation period, the cell suspensions are treated with chloramine-T to decarboxylate