A nondestructive method for the measurement of radioactive 14CO2 in blood

✍ Scribed by F.J. Casado; J.A. Fernández-López; J.M. Argilés; M. Alemany

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 342 KB
• Volume: 172
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(88)90476-9

✦ Synopsis

A method for estimation of 14CO2 present in blood and tissular samples is described. It is basically based on the introduction of large amounts of a gas mixture (95% O2, 5% CO2) in the samples which serves to remove the CO2 label by gas dilution. The gas phase is later captured in scintillation vials containing an organic-soluble base that retains the carbon label. The results obtained by means of this methodology show much better recoveries for blood samples than those obtained when the classic acid-diffusion method is used. In addition, it is a very fast procedure which does not alter the pH or protein integrity of the biological sample.