๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A simple assay for the quantitative evaluation of Phospholipase D activity

โœ Scribed by Rosanna Cardillo; Paola D'Arrigo; Lorenzo Ferra; Valentine Piergianni; Domenico Scarcelli; Stefano Servi

Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
252 KB
Volume
7
Category
Article
ISSN
0951-208X

No coin nor oath required. For personal study only.

โœฆ Synopsis

We describe the preparation of phosphatidyl p-nitrophenol (PpNP) and its use as a substrate in an assay for the quantitative evaluation of Phospholipase D. The solution of the substrate in Tris HCl buffer, pH 8, and Triton X is mixed directly with an aliquot of fermentation broth and the absorbance of the solution read at 405 nm. The method is rapid, sensitive and inexpensive.

๐Ÿ“œ SIMILAR VOLUMES

A Spectrophotometric Assay for the Trans
A Spectrophotometric Assay for the Transphosphatidylation Activity of Phospholipase D Enzyme
โœ Tairo Hagishita; Masanobu Nishikawa; Tadashi Hatanaka ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 77 KB

We developed a specific spectrophotometric assay for the quantitative determination of phospholipase D-catalyzed transphosphatidylation activity. The assay measures p-nitrophenol liberated by phospholipase D-catalyzed reaction of phosphatidyl-p-nitrophenol and ethanol in an aqueous-organic emulsion

Conductimetric Assays for the Hydrolase
Conductimetric Assays for the Hydrolase and Transferase Activities of Phospholipase D Enzymes
โœ M. Mezna; A.J. Lawrence ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 484 KB

Measurement of solution electrical conductance (conductimetry) is a simple direct assay method for the protogenic, hydrolytic reactions catalyzed by all phospholipase enzymes. The technique is especially suitable for assay of phospholipase D (PLD) enzymes where cleavage of zwitterionic substrates re