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A Fluorescent Assay for Agonist-Activated Phospholipase D in Mammalian Cell Extracts

✍ Scribed by K.M. Ella; G.P. Meier; C.D. Bradshaw; K.M. Huffman; E.C. Spivey; K.E. Meier

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
713 KB
Volume
218
Category
Article
ISSN
0003-2697

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A nonradioactive assay for the investigation of phospholipase D (PLD) activity in cardiac membranes has been developed. A fluorescent derivative of phosphatidylcholine [2-decanoyl-1-(O-(11-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3proprionyl)amino) undecyl) sn-glycero-3-phosphocholin