A Short, Novel, and Cheaper Procedure for Oligonucleotide Synthesis Using Automated Solid Phase Synthesizer
β Scribed by Song, Quanlai; Wang, Zhiwei; Sanghvi, Yogesh S.
- Book ID
- 115467993
- Publisher
- Taylor and Francis Group
- Year
- 2003
- Tongue
- English
- Weight
- 240 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0732-8311
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Automated solid phase synthesis of 2'-deoxyribose oligonucleotides; d(Tp)7T, d(Tps)TT and d(Tp)3(CBZp)2(Tp)2T, was achieved directly in 99% overall yields using a novel silyl linkage to a CPG silica support cleavable within seconds at room temperature using TBAF.
## Abstract Various bifunctional aminoβprotecting groups such as the phthaloyl, succinyl, and glutaryl group were investigated as potential linker molecules for attachment to solidβsupport materials. Pentaneβ1,3,5βtricarboxylic acid 1,3βanhydride (16) offered the best properties and reacted with th