𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A sensitive radioisotope assay for pectin methyl esterase activity

✍ Scribed by Yoram Milner; Gad Avigad

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
292 KB
Volume
51
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

The preparation of polygalacturonic acid ["Cl-labeled methyl ester (pectic acid ester) is described. This labeled polysaccharide is employed as the substrate in a simple, sensitive, and rapid assay procedure for measuring pectin methyl esterase activity.

πŸ“œ SIMILAR VOLUMES

A rapid assay for insect juvenile hormon
A rapid assay for insect juvenile hormone esterase activity
✍ Bruce D. Hammock; Thomas C. Sparks πŸ“‚ Article πŸ“… 1977 πŸ› Elsevier Science 🌐 English βš– 429 KB

A rapid new assay is proposed for analyzing insect juvenile hormone esterase activity. The assay is based on > 99% of radiolabeled juvenile hormone being extracted into an isooctane phase while > 9% of the juvenile hormone acid remains in the basic aqueous-methanol phase. The assay is more rapid and

A sensitive spectrophotometric assay for
A sensitive spectrophotometric assay for guanase activity
✍ Takao Ando; Toshiharu Muraoka; Hiromichi Okuda πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 496 KB

A highly sensitive and accurate spectrophotometric method was developed for determination of guanase activity with guanine as substrate. The assay is based on the oxidative coupling of 3-methyl-2benzothiazolinone hydrazone and N,Ndiethylaniline. Xanthine formed from guanine by guanase is oxidized to

A sensitive method for the assay of hyal
A sensitive method for the assay of hyaluronidase activity
✍ Leslie C. Benchetrit; Sham L. Pahuja; Ernest D. Gray; Ronald D. Edstrom πŸ“‚ Article πŸ“… 1977 πŸ› Elsevier Science 🌐 English βš– 417 KB

A spectrophotometric method for the assay of hyaluronidase activity, based on the binding of a carbocyanine dye (l-ethyl-2-[3-( l-ethyl-naphtho[l,M] to undegraded substrate, is described. The binding results in a spectral shift with an absorbance maximum at 640 nm which is propo~ional to the amount

A Sensitive and Selective High-Throughpu
A Sensitive and Selective High-Throughput Screening Fluorescence Assay for Lipases and Esterases
✍ Erich Nyfeler; Johann Grognux; Denis Wahler; Jean-Louis Reymond πŸ“‚ Article πŸ“… 2003 πŸ› John Wiley and Sons 🌐 German βš– 128 KB

## Abstract Long‐chain fatty acid esters of 7‐(3,4‐dihydroxybutyloxy)‐2__H__‐1‐benzopyran‐2‐one (**6**) such as octanoate **2a** are shown to be exceptionally sensitive and selective fluorogenic substrates for lipases and esterases. Umbelliferone (**8**) is released upon hydrolysis of the ester fun