✦ LIBER ✦

A rapid assay for insect juvenile hormone esterase activity

✍ Scribed by Bruce D. Hammock; Thomas C. Sparks

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 429 KB
Volume: 82
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90196-8

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✦ Synopsis

A rapid new assay is proposed for analyzing insect juvenile hormone esterase activity. The assay is based on > 99% of radiolabeled juvenile hormone being extracted into an isooctane phase while > 9% of the juvenile hormone acid remains in the basic aqueous-methanol phase. The assay is more rapid and less expensive than conventional chromatographic assays while yielding almost identical values of similar precision. This is the first rapid assay proposed for isomerically pure, commercially available juvenile hormone I.

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