A rapid partition assay for routine analysis of juvenile hormone release by insect corpora allata

✍ Scribed by René Feyereisen; Stephen S. Tobe

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 313 KB
• Volume: 111
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90575-3

✦ Synopsis

A partition assay is described which allows a rapid determination of juvenile hormone release by insect corpora allata. Newly synthesized and released methyl-labeled C,, juvenile hormone is quantitatively extracted into an isooctane phase, whereas precursor methyl-labeled L-methionine remains in the incubation medium. When used in conjunction with appropriate blank assays, this method offers both accuracy and speed necessary for routine analysis of corpora allata activity.