✦ LIBER ✦

Gas chromatography-mass spectroscopy analysis of juvenile hormone released by insect corpora allata

✍ Scribed by Bernard mauchamp; Franck Couillaud; Christian Malosse

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 396 KB
Volume: 145
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90358-6

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✦ Synopsis

Corpora allata incubated in appropriate medium release several compounds including juvenile hormones. Juvenile hormones (14C labeled or unlabeled) were extracted with hexane and directly analyzed by gas chromatography-mass spectroscopy. This method allowed the qualitative and quantitative analysis of total released juvenile hormone. It could also be used as a routine assay for evaluation of corpus allatum activity. Data obtained by this method were compared to those obtained by radiochemical assay.

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