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A sensitive method for the assay of hyaluronidase activity

✍ Scribed by Leslie C. Benchetrit; Sham L. Pahuja; Ernest D. Gray; Ronald D. Edstrom

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
417 KB
Volume
79
Category
Article
ISSN
0003-2697

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✦ Synopsis

A spectrophotometric method for the assay of hyaluronidase activity, based on the binding of a carbocyanine dye (l-ethyl-2-[3-( l-ethyl-naphtho[l,M]

to undegraded substrate, is described. The binding results in a spectral shift with an absorbance maximum at 640 nm which is propo~ional to the amount of hyafuronic acid within a prescribed range. The end products of hyaluronidase activity do not cause a shift in the dye spectrum. The method allows the determination of an amount of hyaluronidase equivalent to 0.00005 NF unit.

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