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A fluorometric method for assay of RNase activity

โœ Scribed by Richard C. Kamm; Albert G. Smith; Harold Lyons

Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
215 KB
Volume
37
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Most of the methods currently available for the determination of RNase activity are dependent on the measurement of acid-soluble products released 'by hydrolysis of RNA. The major advantages of these methods are that they are sensitive, reproducible, and convenient for large numbers of samples (l-3). The major disadvantage, however is that there is danger of nonenzymic degradation of the RNA during the incubation, requiring careful temperature control and rapid handling (1, 3).

LePecq, Tot, and Paoletti (4) recently reported that the dye ethidium bromide forms a fluorescent complex with nonhydrolyzed RNA, but fails to fluoresce in the presence of hydrolyzed RNA, thereby forming the basis of a simple, rapid method for the determination of RNase activity by observation of the change in fluroescence as a standard amount of RNA is hydrolyzed by the RNase present in the sample.

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