𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A Sensitive and Selective High-Throughput Screening Fluorescence Assay for Lipases and Esterases

✍ Scribed by Erich Nyfeler; Johann Grognux; Denis Wahler; Jean-Louis Reymond

Publisher
John Wiley and Sons
Year
2003
Tongue
German
Weight
128 KB
Volume
86
Category
Article
ISSN
0018-019X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Long‐chain fatty acid esters of 7‐(3,4‐dihydroxybutyloxy)‐2__H__‐1‐benzopyran‐2‐one (6) such as octanoate 2a are shown to be exceptionally sensitive and selective fluorogenic substrates for lipases and esterases. Umbelliferone (8) is released upon hydrolysis of the ester function in 2a in the presence of bovine serum albumin and sodium periodate. These substrates are at least by one order of magnitude more sensitive to lipases than the commercial fluorogenic substrate 4‐methylumbelliferyl heptanoate. Furthermore, they are stable to a broad range of pH‐induced‐ and thermal‐hydrolysis conditions and do not react with non‐catalytic proteins such as bovine serum albumin (BSA).

📜 SIMILAR VOLUMES

New Monofunctionalized Fluorescein Deriv
New Monofunctionalized Fluorescein Derivatives for the Efficient High-Throughput Screening of Lipases and Esterases in Aqueous Media
✍ Yongzheng Yang; Peter Babiak; Jean-Louis Reymond 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 German ⚖ 317 KB

## Abstract Monoalkylation or acylation of fluorescein (**1**) with various acyloxymethyl or acyl halides afforded, respectively, a series of ether‐ (**2**) and ester‐functionalized (**3**) fluorogenic probes. The highly reactive and water‐soluble substrates release fluorescein (**1**) upon reactio

A High-Throughput Screening Assay for Am
A High-Throughput Screening Assay for Amino Acid Decarboxylase Activity
✍ Rosario Médici; Pablo Domínguez de María; Linda G. Otten; Adrie J. J. Straathof 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 438 KB 👁 2 views

## Abstract The development of sensitive and easy‐to‐apply high‐throughput screening methods is a common need in modern biocatalysis. With these powerful analytical tools in hands, chemists can easily assess enzyme libraries to identify either novel biocatalysts or improved mutants. Within biocatal

A Practical NMR-Based High-Throughput As
A Practical NMR-Based High-Throughput Assay for Screening Enantioselective Catalysts and Biocatalysts
✍ Manfred T. Reetz; Andreas Eipper; Patrick Tielmann; Richard Mynott 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 148 KB 👁 2 views

Two NMR-based approaches for highthroughput screening of enantioselective catalysts and biocatalysts are described. One version makes use of pseudo-enantiomers or pseudo-meso-compounds based on 13 C-labeling. A throughput of at least 1400 ee determinations per day is possible by using an appropriate