The preparation of polygalacturonic acid ["Cl-labeled methyl ester (pectic acid ester) is described. This labeled polysaccharide is employed as the substrate in a simple, sensitive, and rapid assay procedure for measuring pectin methyl esterase activity.
A pH-Independent Assay for Pectin Methyl Esterase for Use in Column Chromatography
โ Scribed by G.P. Mcmillan; D.J. Johnston; J.B. Morel; M.C.M. Perombelon
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 250 KB
- Volume
- 209
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
the molecular mass of the labeled molecule, the greater BODIPY-a-casein is a new fluorescent protein subthe motion (rotation) will be and the lower the emitted strate designed for fluorescence polarization studies fluorescence polarization value. When a relatively large to measure proteolytic activi
A general procedure is described for developing HPLC-based assays for nucleotidases by using a 20-mm C-18 reversed-phase column. The method is applied on the determination of pyrimidine 5'-nucleotidase activity in human erythrocytes lysate and plasma. The activity is detected using isocratic conditi
## Abstract A new design for an electron capture detector cell is described. The cell is compatible with the requirements of high resolution capillary columns and is shown to be useful in applications that require high analysis speed. A unique method of sample introduction reduces the problems of s
## ~~ ~~ B. Brule, Application de la chromatographie sur gel permeable (GPC) a la caracteristion de bitumes routiers et de leur susceptibilite auvieissementartificiel.Bull.1iaisonP. etCh.128Nov-Dec1983.