A rapid and easy method for DNA recovery from agarose gels using Wizard minicolumns
โ Scribed by Patrice R.A. Wolff; Roger Hull
- Book ID
- 114173663
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 278 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0168-9525
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A simple and reproducible procedure for the recovery of plasmid DNA is described. The method was standardised for the purification of plasmids from Giuconobacfer oxydans ATCC9937 . The protocol is based on the use of glass microfibre filter paper for entrapment of DNA and its subsequent recovery by
## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %