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A preparation and purification of [1-14C]acetylcarnitine

✍ Scribed by Grattan Roughan; John Browse


Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
341 KB
Volume
207
Category
Article
ISSN
0003-2697

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✦ Synopsis


[1-14C]Acetylcarnitine was prepared from [1-14C]acetate and L-carnitine using acetyl-CoA synthetase and carnitine acetyltransferase. The product was purified by ion-exchange and thin-layer chromatography. Conversion of [1-14C]acetate to [1-14C]acetylcarnitine was better than 90% and overall recovery of the pure product was greater that 80%.


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