Propylproline and N-demethyl-lincomycin, two i n t e r m e d i a t e s i n l i n c o m y c i n b i o s y n t h e s i s , were prepared i n r a d i o l a b e l e d form. [\*\*C]-labeled N-demethyllincomycin was s y n t h e s i z e d by a r e s t i n g cell suspension of a lincomycin n e g a t i v e
A preparation and purification of [1-14C]acetylcarnitine
β Scribed by Grattan Roughan; John Browse
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 341 KB
- Volume
- 207
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
β¦ Synopsis
[1-14C]Acetylcarnitine was prepared from [1-14C]acetate and L-carnitine using acetyl-CoA synthetase and carnitine acetyltransferase. The product was purified by ion-exchange and thin-layer chromatography. Conversion of [1-14C]acetate to [1-14C]acetylcarnitine was better than 90% and overall recovery of the pure product was greater that 80%.
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