A reusable piezoelectric immunosensor was developed for the detection of purified human erythrocytes and erythrocytes in whole human blood. Three different methods for immobilization of anti-glycophorin A antibody to the gold electrode were tested. Coating the electrode with polyethylenimine gave th
A Piezoelectric Immunosensor for the Detection of Cocaine
β Scribed by Bassam S. Attili; Ahmad A. Suleiman
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 68 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0026-265X
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β¦ Synopsis
A piezoelectric crystal immunosensor has been developed for the detection and determination of cocaine in the concentration range 10 -300 mg/liter (part per billion) with a relative SD of 10 -19%. Benzoylecgonine antibody, the major metabolite of cocaine, was layered onto the gold electrodes of a 10 MHz piezoelectric crystal, which was precoated with protein G. The protein G immobilization procedure showed the best results in terms of sensitivity, stability, and reproducibility. The piezoelectric immunosensor technology demonstrated in this work shows a successful application which could have a tremendous impact on drug of abuse monitoring.
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A highly sensitive piezoelectric crystal immunosensor has been developed for the detection of human complement C4. Three methods for immobilization of anti-C4 antibody on the gold electrodes were employed. The immobilization method with protein A method gave the best results in terms of sensitivity,
The long-term stability and reusability of a recently developed piezoelectric immunosensor for the detection of purified human erythrocytes and erythrocytes in whole human blood was studied. Using polyethyleneimine-anti-glycophorin A antibody-coated gold electrodes a long-term stability of 10 weeks