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A piezoelectric immunosensor for complement C4 using protein A oriented immobilization of antibody

✍ Scribed by Ren-Jun Pei; Ji-Ming Hu; Yi Hu; Yun'e Zeng

Publisher
Wiley (John Wiley & Sons)
Year
1998
Tongue
English
Weight
177 KB
Volume
73
Category
Article
ISSN
0268-2575

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✦ Synopsis

A highly sensitive piezoelectric crystal immunosensor has been developed for the detection of human complement C4. Three methods for immobilization of anti-C4 antibody on the gold electrodes were employed. The immobilization method with protein A method gave the best results in terms of sensitivity, stability and reproducibility. The coated crystal with either polyethyleneimine adhesion, followed by a glutaraldehyde cross-linking method, or physical adsorption method, gave inferior results. A thin protein A membrane was formed on the gold electrodes so that C4 antibody could be e †ectively selfassembled, as an orderly, closely arranged and oriented antibody membrane on the protein A layer. Under the optimized experimental conditions, the coated crystal had good response to C4 in the range 5 ] 10~4 to 1 ] 10~2 g dm~3. Other proteins in human serum did not interfere with the detection. The crystal could be regenerated nearly 10 times.

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