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Long-term stability and improved reusability of a piezoelectric immunosensor for human erythrocytes

✍ Scribed by Bernd König; Michael Grätzel

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
421 KB
Volume
280
Category
Article
ISSN
0003-2670

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✦ Synopsis

The long-term stability and reusability of a recently developed piezoelectric immunosensor for the detection of purified human erythrocytes and erythrocytes in whole human blood was studied. Using polyethyleneimine-anti-glycophorin A antibody-coated gold electrodes a long-term stability of 10 weeks was found when stored dry at room temperature. Storage at 4°C did not improve this stability. To improve the reusability a synthetic peptide corresponding to the human glycophorin A sequence of the amino acids 27-39 was used for competition with the bound antigen. With this technique the number of regenerations could be increased from five to twelve without a decrease in activity.

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