𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Development of a piezoelectric immunosensor for the detection of human erythrocytes

✍ Scribed by Bernd König; Michael Grätzel

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
445 KB
Volume
276
Category
Article
ISSN
0003-2670

No coin nor oath required. For personal study only.

✦ Synopsis

A reusable piezoelectric immunosensor was developed for the detection of purified human erythrocytes and erythrocytes in whole human blood. Three different methods for immobilization of anti-glycophorin A antibody to the gold electrode were tested. Coating the electrode with polyethylenimine gave the best results in terms of stability, sensitivity and reproducibility. The other two methods, coating the electrode with y-aminopropyltriethoxysilane and protein A, gave inferior results. Applying an anti-glycophorin A antibody layer via polyethylenimine immobilization on a lo-MHz AT-cut crystal resulted in a linear frequency change for 1 X lo34 X lo4 cells ml-'.

📜 SIMILAR VOLUMES

A Piezoelectric Immunosensor for the Det
A Piezoelectric Immunosensor for the Detection of Cocaine
✍ Bassam S. Attili; Ahmad A. Suleiman 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 68 KB

A piezoelectric crystal immunosensor has been developed for the detection and determination of cocaine in the concentration range 10 -300 mg/liter (part per billion) with a relative SD of 10 -19%. Benzoylecgonine antibody, the major metabolite of cocaine, was layered onto the gold electrodes of a 10

Long-term stability and improved reusabi
Long-term stability and improved reusability of a piezoelectric immunosensor for human erythrocytes
✍ Bernd König; Michael Grätzel 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 421 KB

The long-term stability and reusability of a recently developed piezoelectric immunosensor for the detection of purified human erythrocytes and erythrocytes in whole human blood was studied. Using polyethyleneimine-anti-glycophorin A antibody-coated gold electrodes a long-term stability of 10 weeks

Electrochemical immunosensor for the det
Electrochemical immunosensor for the detection of atrazine
✍ Miguel A. López; Fidel Ortega; Elena Domínguez; Ioanis Katakis 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 101 KB 👁 1 views

An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy) 2 Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the o

Self-Assembled Monolayer-Based Piezoelec
Self-Assembled Monolayer-Based Piezoelectric Crystal Immunosensor for the Quantification of Total Human Immunoglobulin E
✍ Xiaodi Su; Fook Tim Chew; Sam F.Y. Li 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 78 KB

This work presents a piezoelectric (Pz) immunosensor for the quantification of total human IgE in serum samples. The anti-human IgE is deposited on the surface of the 10 M Hz AT-cut gold coated crystal resonator by self-assembled technique, and serves as a receptor layer. The highly ordered self-ass