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Electrochemical immunosensor for the detection of atrazine

✍ Scribed by Miguel A. López; Fidel Ortega; Elena Domínguez; Ioanis Katakis


Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
101 KB
Volume
11
Category
Article
ISSN
0952-3499

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✦ Synopsis


An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy) 2 Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H 2 O 2 at 0.1 V (vsAg/AgCl).


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