An electrochemical enzyme immunoassay for polychlorinated biphenyls (PCB) using carbon-based screen-printed disposable electrodes as the solid-phase for reagent immobilization and also as the signal transducer has been optimized. Horseradish peroxidase (HRP) was conjugated with Ag or Ab and used as
Electrochemical immunosensor for the detection of atrazine
✍ Scribed by Miguel A. López; Fidel Ortega; Elena Domínguez; Ioanis Katakis
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 101 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0952-3499
No coin nor oath required. For personal study only.
✦ Synopsis
An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy) 2 Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H 2 O 2 at 0.1 V (vsAg/AgCl).
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