Portable Immunosensor for the Fast Amperometric Detection of Anti-Hantavirus Antibodies

✍ Scribed by Srinivas Vetcha; Ihab Abdel-Hamid; Plamen Atanasov; Dmitri Ivnitski; Ebtisam Wilkins; Brian Hjelle

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 479 KB
• Volume: 12
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/1521-4109(200009)12:13<1034::aid-elan1034>3.0.co;2-l

✦ Synopsis

An amperometric immunosensor based on a recombinant nucleocapsid antigen has been developed for fast determination of antibodies against Sin Nombre (SN) virus in human blood serum. This approach combines the advantages of utilization of highly dispersed immunosorbent, with highly sensitive electrochemical detection of enzyme-marker, and ¯ow-injection scheme of immunoassay. A main bene®t of recombinant nucleocapsid antigen is that antigen preparation and standardization is easy. The complete immunoassay can be performed in 24 minutes. The short overall assay time could be a major advantage in the ®eld of diagnostic screening of infectious diseases in mobile laboratories and small hospitals. The immunosensor has been successfully used in the determination of anti-Hantavirus IgG in human blood plasma and is easily adaptable for the detection of other types of viral infections.