A pH-metrical method of determining glucose-6-phosphatase activity
β Scribed by Marat B. Murataliev; Evgeni N. Vulfson
- Book ID
- 107712113
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 280 KB
- Volume
- 151
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A sensitive and rapid spectrophotometric method for determination of glucose-6-phosphatase activity is described. Glucose formed by the enzyme is oxidized by glucose oxidase to gluconolactone and hydrogen peroxide. The latter, phenol, and 4-aminoantipyrine are converted by peroxidase to quinoneimine
We present a method to determine glucose 6-phosphate activity. This assay measures the rate of glucose released in the glucose-6-phosphatase reaction. The glucose is oxidized to beta-D-gluconolactone by glucose dehydrogenase in a coupled reaction that uses NAD(P)+. The determination is rapid, reprod
A method is described for measuring the activity of glucose-6-phosphatase (EC 3.1.3.9) in rat liver. [U-W]Glucose 6-phosphate, as substrate, is converted by the enzyme to [Ylglucose and inorganic phosphate. The addition of ZnSO, and Ba(OH), at the end of the reaction precipitates phosphate and the u