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A spectrophotometric method for the determination of glucose-6-phosphatase activity

✍ Scribed by P. Gierow; B. Jergil

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
361 KB
Volume
101
Category
Article
ISSN
0003-2697

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✦ Synopsis

A sensitive and rapid spectrophotometric method for determination of glucose-6-phosphatase activity is described. Glucose formed by the enzyme is oxidized by glucose oxidase to gluconolactone and hydrogen peroxide. The latter, phenol, and 4-aminoantipyrine are converted by peroxidase to quinoneimine. The formation of quinoneimine is followed directly on a spectrophotometer at 510 nm. The method described is as sensitive and accurate as conventional assays based on determination of phosphate released, and also works well in phosphate buffers.

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