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Determination of glucose-6-phosphatase activity using the glucose dehydrogenase-coupled reaction

✍ Scribed by María Alegre; Carlos J. Ciudad; Cristina Fillat; Joan J. Guinovart

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
444 KB
Volume
173
Category
Article
ISSN
0003-2697

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✦ Synopsis

We present a method to determine glucose 6-phosphate activity. This assay measures the rate of glucose released in the glucose-6-phosphatase reaction. The glucose is oxidized to beta-D-gluconolactone by glucose dehydrogenase in a coupled reaction that uses NAD(P)+. The determination is rapid, reproducible, and does not require withdrawal, precipitation, centrifugation, or neutralization steps. This method provides a simple resolution to the problem of the nonspecific appearance of Pi, which is especially important in studies of regulation of glucose-6-phosphatase performed in the presence of ATP.

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