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A method for the determination of glucose-6-phosphatase activity in rat liver with [U-14C]glucose 6-phosphate as substrate

✍ Scribed by S.A. Kitcher; K. Siddle; J.P. Luzio

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 414 KB
Volume: 88
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90395-0

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✦ Synopsis

A method is described for measuring the activity of glucose-6-phosphatase (EC 3.1.3.9) in rat liver. [U-W]Glucose 6-phosphate, as substrate, is converted by the enzyme to [Ylglucose and inorganic phosphate. The addition of ZnSO, and Ba(OH), at the end of the reaction precipitates phosphate and the unreacted [Wlglucose 6-phosphate, whereas [Ylglucose is not precipitated. After centrifugation, the amount of [Ylglucose formed is determined in a liquid scintillation counter.

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