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A continuous spectrophotometric assay for the enzymatic marker glucose 6-phosphatase

โœ Scribed by Maria Stio; Paolo Vanni; Giancarlo Pinzauti

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
484 KB
Volume
174
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A continuous spectrophotometric assay for glucose 6-phosphatase is described. The method uses glucose dehydrogenase and mutarotase as ancillary enzymes. Glucose 6-phosphatase activity is measured by following NADH formation at 340 nm. The method is linear, at least up to 38 mU in the test which corresponds to a AE of 0.24 mini', when the enzyme is assayed in a microsomal fraction. We also discuss the method's suitability for subcellular fractionation. No other continuous assay for this important enzymatic marker of the endoplasmic reticulum is currently available.

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