Determination of rat liver microsomal glucose-6-phosphatase activity: Study of citrate and G-6-P inhibition
✍ Scribed by Eugene S. Baginski; Piero P. Foà; Bennie Zak
- Book ID
- 107710817
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 340 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0003-2697
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Spontaneous S6 phosphatase activities dephosphorylating Ser(P)-235 and Ser(P)-236 of the ribosomal protein S6 were measured and compared in microsomes and cytosol of rat liver. The substrate used, small (40S) ribosomal subunits 32P-labelled in vitro by protein kinase A, contained phosphorylated S6 (
A method is described for measuring the activity of glucose-6-phosphatase (EC 3.1.3.9) in rat liver. [U-W]Glucose 6-phosphate, as substrate, is converted by the enzyme to [Ylglucose and inorganic phosphate. The addition of ZnSO, and Ba(OH), at the end of the reaction precipitates phosphate and the u