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A new colorimetric method for the determination of 6-aminopenicillanic acid

✍ Scribed by Joseph M. Kornfeld

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
470 KB
Volume
86
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new, sensitive calorimetric method for the estimation of 6-aminopenicillanic acid is described. The procedure is based upon formation of a 2,4-pentanedione derivative of 6-aminopenicillanic acid followed by a second reaction with p-dimethylaminobenzaldehyde (Ehrlich's reagent), resulting in a red product which absorbs at 538 nm. The absorbance response is linear from 0 to 350 pg of 6-aminopenicillanic acid. Penicillins do not interfere with the assay, but 6-aminopenicilloic acid does.

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