✍ Scribed by Mörsel, J.-Th. ;Heyer, R.
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The quantitative determination of lipids is, a universal and repeating problem of analytical chemistry in different fields, for example food-or biochemistry. Important criterions to value methods are the time needed for analysis, reproducibility and detection limit. The determination of glycerides can be performed by classical methods, for instance by the WEIBULL-STOLDT method [I] or by extraction. HPLC and GLC can be used successfully, too. All these methods are expensive of time or equipment.
In this paper a colorimetric method is reported characterized by good reproducibility, great sensitivity and little time needed to perform. The determination, first time reported by FLETCHER [2] is based on the oxidation of glycerol after saponification of the lipid with periodate. The formaldehyde produced is determined by coupling a dye in a HANTZSCH condensation with ammonium ions and 2,4-pentadione. The 3,5-diacetyl-1,Cdihydrolutidin formed is measured in a photometer at 410nm.
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