A colorimetric method for the determination of hydroxamic acid by iodine oxidation
β Scribed by Kyoichi Kobashi; Kazuo Sakaguchi; Sachiko Takebe; Kohei Hosaka
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 433 KB
- Volume
- 146
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
β¦ Synopsis
A new calorimetric method for the determination of hydroxamic acid is described. Hydroxamic acid was oxidized quantitatively by iodine to produce nitrous acid, which was thereafter determined according to the diazocoupling reaction. This method is sensitive to as little as 5 nmol of hydroxamic acid, and the calibration curve is linear up to 50 nmol. Using this method, acyl-CoAs were determined after conversion to hydroxamic acid by the addition of hydroxylamine. The present method is applicable to the determination of free fatty acids which are activated by acyl-CoA synthetase.
π SIMILAR VOLUMES
A new, sensitive calorimetric method for the estimation of 6-aminopenicillanic acid is described. The procedure is based upon formation of a 2,4-pentanedione derivative of 6-aminopenicillanic acid followed by a second reaction with p-dimethylaminobenzaldehyde (Ehrlich's reagent), resulting in a red
The purpose of this study was to develop a procedure for the quantitative analysis of the salts of the solanaceous alkaloids in the presence of their hydrolysis products. The salts taken were atropine sulfate, homatropine hydrobromide, homatropine methylbromide, scopolamine hydrobromide, and scopola
The quantitative determination of lipids is, a universal and repeating problem of analytical chemistry in different fields, for example food-or biochemistry. Important criterions to value methods are the time needed for analysis, reproducibility and detection limit. The determination of glycerides c