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A microtiter plate-based assay for phosphoenolpyruvate carboxylase

✍ Scribed by William Cockburn; Garry C. Whitelam; Stephen P. Slocombe; Raymond A. McKee

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
325 KB
Volume
189
Category
Article
ISSN
0003-2697

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✦ Synopsis

A sensitive, quantitative assay for phosphenolpyruvate carboxylase which utilizes microtiter plates is described. The assay depends upon the production of a colored compound in the reaction between oxaloacetate, the product of the phosphoenolpyruvate reaction, and the dye Fast Violet B. The method is particularly appropriate for monitoring chromatographic eluates and its utility for this purpose is demonstrated by the detection of phosphoenolpyruvate carboxylase in fractions of crude maize extract separated by size-exclusion chromatography.

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