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A Microtiter Plate Assay for Polyglutamine Aggregate Extension

✍ Scribed by Valerie Berthelier; J.Bradley Hamilton; Songming Chen; Ronald Wetzel

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
114 KB
Volume
295
Category
Article
ISSN
0003-2697

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A sensitive, quantitative assay for phosphenolpyruvate carboxylase which utilizes microtiter plates is described. The assay depends upon the production of a colored compound in the reaction between oxaloacetate, the product of the phosphoenolpyruvate reaction, and the dye Fast Violet B. The method i