We describe a simple endpoint method for the determination ofN-acetyl-@ghtcosaminidase (NAGase; EC 3.2.1.30). NAGase uses a fluorogenic substrate, 4-methylumbelliferyl-IV-acetyl-@tx-ghtcosaminide, at pH 4.6, liberating the fluorescent 4-methylumbelliferone. The method is reproducible and fast both a
A Colorimetric Microtiter Plate Assay for Lysostaphin Using a Hexaglycine Substrate
β Scribed by S.A. Kline; J. Delaharpe; P. Blackburn
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 279 KB
- Volume
- 217
- Category
- Article
- ISSN
- 0003-2697
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