✦ LIBER ✦

A microtiter plate-based assay method to determine fat quality

✍ Scribed by Mark Bertram; Corrine Manschot-Lawrence; Eckhard Flöter; Uwe T. Bornscheuer

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 329 KB
Volume: 109
Category: Article
ISSN: 1438-7697
DOI: 10.1002/ejlt.200600193

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A microtiter plate (MTP) method was developed to determine the quality of fats that are used in large‐scale processing using lipase catalysis. Two assay formats were followed: In the first approach, the fats were interesterified with p‐nitrophenol laurate using a lipase from Thermomyces lanuginosa; in the second approach, pH indicators were added to the fat samples containing lipase. A blind study using 29 fats showed that the MTP method using p‐nitrophenol as pH indicator allowed a rapid and reliable assignment of bad fats and an acceptable differentiation between fats of moderate and good quality.

📜 SIMILAR VOLUMES

A noninvasive method to determine fat co

A noninvasive method to determine fat content in small fish based on swim bladder size estimation

✍ Alexander Kotrschal; Barbara Fischer; Barbara Taborsky 📂 Article 📅 2011 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 210 KB

## Abstract The presence of fat stores in fish is widely used as a correlate of fish health and fitness. Techniques to measure fat content with some accuracy are available for medium‐sized and large fish, but apart from morphometric indices, a noninvasive method to determine fat content in small fi

Adaptation of the Periodate-Resorcinol M

Adaptation of the Periodate-Resorcinol Method for Determination of Sialic Acids to a Microassay Using Microtiter Plate Reader

✍ V.P. Bhavanandan; M. Sheykhnazari 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 318 KB

A silica gel plate-based qualitative ass

A silica gel plate-based qualitative assay for acetylcholinesterase activity: A mass method to screen for potential inhibitors

✍ John S. Kiely; Walter H. Moos; Micheal R. Pavia; Roy D. Schwarz; Gail L. Woodard 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 423 KB

A procedure for the qualitative assessment of inhibitory activity towards acetylcholinesterase for a given compound is described. Solutions of the compounds of interest are spotted on silica gel TLC plates in a matrix pattern. The silica gel plate is sprayed with a solution of acetylthiocholine iodi