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A micromethod for the isolation of drugs from blood using Amberlite XAD-2

✍ Scribed by Schlicht, H.J. ;Gelbke, H.P.

Book ID
104660676
Publisher
Springer-Verlag
Year
1978
Tongue
English
Weight
370 KB
Volume
81
Category
Article
ISSN
1437-1596

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✦ Synopsis

The extraction of drugs from small blood samples (1 ml or less) for subsequent quantitative determination is described. Isolation was carried out by adsorption of the drags to Ambeflite XAD-2 resin utilizing a batch procedure that enabled the simultaneous extraction of up to 200 samples in approx. 5 hours. A new desorption technique yielded extracts of high purity that could be used directly for gas chromatographic or radioimmunological determinations, even if hemolyzed or putrid blood was to be examined. The following 26 substances were quantitated after addition to postmortem blood specimens at concentrations of 1-10 /~g/ml:

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