The extraction of drugs from small blood samples (1 ml or less) for subsequent quantitative determination is described. Isolation was carried out by adsorption of the drags to Ambeflite XAD-2 resin utilizing a batch procedure that enabled the simultaneous extraction of up to 200 samples in approx. 5
Isolation of drugs from blood by column chromatography on amberlite XAD-2
β Scribed by H. P. Gelbke; T. H. Grell; Gg. Schmidt
- Book ID
- 104743477
- Publisher
- Springer-Verlag
- Year
- 1978
- Tongue
- English
- Weight
- 472 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0340-5761
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β¦ Synopsis
An investigation was carried out on the isolation of 28 drugs from blood by column chromatography on Amberlite XAD-2. The following substances were added to postmortem blood specimens at concentrations generally of I-i0 ~g/ml:
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The potentials of XAD-columns for the isolation of quaternary ammonium compounds from aqueous media have been investigated. When adequate amounts of counter ions (perchlorate, chloride, phosphate, nitrate) were added to the aqueous sample, to the column pretreatment fluid and to the aqueous washing
The method of adsorption of drugs on Amberlite XAD-2 resin, followed by differential elution was under study during 1 1/2 year of routine use. The method allows a separation of acidic and basic drugs and--due to acid hydrolysis step before adsorption--assures better recovery of conjugated and protei