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A micromethod for the isolation of total RNA from adipose tissue

✍ Scribed by Kamran Tavangar; Andrew R. Hoffman; Fredric B. Kraemer

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 687 KB
Volume: 186
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(90)90572-q

No coin nor oath required. For personal study only.

✦ Synopsis

We have developed a simple and rapid procedure for the isolation of total RNA from small amounts of adipose tissue. Using this method, it is possible to obtain quantitative recovery of RNA from less than 300 mg of adipose tissue, with an average yield of 70 micrograms of RNA per gram of adipose tissue. Northern blot analysis of rat epididymal adipose tissue RNA samples was performed using a beta-actin probe and demonstrated that intact total RNA had been isolated. The procedure has been adapted for use in 1.5-ml microcentrifuge Eppendorf tubes, providing a convenient and inexpensive method for the reproducible recovery of intact RNA from sparse samples of adipose tissue.

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