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An improved method for isolating RNA from porcine adipose tissue

โœ Scribed by I. Louveau; S. Chaudhuri; T.D. Etherton

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
451 KB
Volume
196
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 M guanidium thiocyanate, 25 mM sodium citrate, 0.5% Sarcosyl, 0.1 M beta-mercaptoethanol, pH 7.0, and then performing two CHCl3 extractions to remove lipid before following the procedure described by P. Chomczynski and N. Sacchi (1987, Anal. Biochem. 162, 156-159). This modification improved the yield of RNA approximately threefold (yield was 88 +/- 7 micrograms total RNA/g of tissue) without affecting RNA quality.

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