Effective Isolation of High-Quality Total RNA from Human Adult Articular Cartilage

✍ Scribed by Louise A. McKenna; Angelika Gehrsitz; Stefan Söder; Wolfgang Eger; Thomas Kirchner; Thomas Aigner

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 159 KB
• Volume: 286
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2000.4779

✦ Synopsis

The isolation of large quantities of good-quality RNA from human articular cartilage has been a long-standing problem for researchers working with human articular cartilage. In this paper we report a protocol which we have developed based on the Qiagen RNeasy procedure to produce high yields of purified, DNA-free RNA from normal and osteosteoarthritic human articular cartilage. The average yield of RNA was 8.39 microg/g (n = 59) for normal and 6.69 microg/g (n = 58) for osteoarthritic cartilage (average ratio OD 260/280 = 1.8-1.9). Quantitative PCR, cDNA array technology, and Northern blot analysis were used to verify the quality of the RNA.