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α2A-Adrenoceptor-specific Stimulation of [35S]GTPγS Binding to Membrane Preparations of Rat Frontal Cortex

✍ Scribed by Oliver Pulges; Ago Rinken

Book ID
106483811
Publisher
Springer
Year
2007
Tongue
English
Weight
350 KB
Volume
33
Category
Article
ISSN
0364-3190

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✍ Juha M. Peltonen; Marjo Pihlavisto; Mika Scheinin 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 99 KB

We measured agonist-stimulated binding of the stable GTP-analog guanosine-5'-O-(3-[35S]thiotriphosphate) ([35S]GTPgammaS) as a functional assay to monitor G-protein activation by recombinant human alpha2-adrenoceptor subtypes alpha2A, alpha2B and alpha2C. (-)-Noradrenaline was a full agonist in all