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Ligand modulation of [35S]GTPγS binding at human α2A, α2B and α2C adrenoceptors

✍ Scribed by Valérie Audinot; Nelly Fabry; Jean-Paul Nicolas; Philippe Beauverger; Adrian Newman-Tancredi; Mark J Millan; Anne Try; Frédéric Bornancin; Emmanuel Canet; Jean A Boutin

Book ID
114283711
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
207 KB
Volume
14
Category
Article
ISSN
0898-6568

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✍ Juha M. Peltonen; Marjo Pihlavisto; Mika Scheinin 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 99 KB

We measured agonist-stimulated binding of the stable GTP-analog guanosine-5'-O-(3-[35S]thiotriphosphate) ([35S]GTPgammaS) as a functional assay to monitor G-protein activation by recombinant human alpha2-adrenoceptor subtypes alpha2A, alpha2B and alpha2C. (-)-Noradrenaline was a full agonist in all