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X-RAY MICROANALYSIS OF ETOPOSIDE-INDUCED APOPTOSIS IN THE PC-3 PROSTATIC CANCER CELL LINE

โœ Scribed by M. Salido; J. Vilches; A. Lopez; G.M. Roomans

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
642 KB
Volume
25
Category
Article
ISSN
1065-6995

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โœฆ Synopsis

Apoptosis comprises a critical intracellular defense mechanism against tumourigenic growth. We have been interested in the relationship between morphological changes and intracellular concentration of several cations after etoposideโ€induced apoptosis in androgenโ€independent prostate cancer cells. SEM and Xโ€ray microanalysis were performed on freezeโ€dried PC3 cells after etoposide treatment, and correlated with the morphological features observed after examination by light and fluorescence microscopy. Cell viability assays were also performed. A significant decrease in intracellular Cl^โˆ’^and K^+^and a progressive increase in Mg^2+^and Na^+^were observed, with parallel changes in cellular volume as cells passed through three morphological stages of apoptosis. The use of EPXRMA made it possible to evaluate alterations in element composition in prostate cancer cell apoptosis and may be a helpful tool for further studies on apoptosis in prostate cancer.

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