Ion transport mediated by osmotically induced chloride channels in HT29 cells was investigated by X-ray microanalysis of cell cultures. The cells were cultured on cellulose nitrate filters, and exposed to isotonic, hypotonic, or hypertonic solutions. Then the NaCl-rich medium was removed by rinsing
X-RAY MICROANALYSIS IN CELL BIOLOGY AND CELL PATHOLOGY
โ Scribed by GODFRIED M. ROOMANS; ANNE VON EULER
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 121 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1065-6995
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โฆ Synopsis
Abstract
Electron probe Xโray microanalysis has been used for the last 25 years by biologists to obtain information about the distribution of elements at the cell and tissue level. During this period, progress has mainly been made through the development of more adequate techniques for specimen preparation (mainly low temperature techniques) and quantitative analysis, so that accurate analysis of the physiologically important cellular ions can be carried out. Use of__in vitro__systems and cell cultures may further increase the number of problems to which Xโray microanalysis can be applied. Among the numerous applications of Xโray microanalysis in cell biology and cell pathology, applications in the areas of epithelial ion transport, the role of calcium in secretory and contractile cells, and the role of ions in cell proliferation and cancer are discussed in more detail.
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