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Cell surface binding of colloidal iron and Limax flavus agglutinin compared by X-ray microanalysis.

✍ Scribed by N. Thomas; P.M. Evans; I. ap Gwynn


Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
265 KB
Volume
17
Category
Article
ISSN
1065-6995

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✦ Synopsis


Abstract

The surface characteristics of tumour cells and T‐cell hybridoma variants were examined by quantitative X‐ray microanalysis using metal‐based probes. Comparisons of populations of cells differing in invasive potential revealed differences in the binding of positive colloidal iron hydroxide, a general probe for anionic sites. Substantial differences between populations were also detected by the binding of Limax flavus agglutinin, which was used in conjunction with fetuin‐gold as a specific marker for sialic acid.