✦ LIBER ✦

Single-cell microinjection technology in cell biology

✍ Scribed by Yan Zhang; Long-Chuan Yu

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 142 KB
Volume: 30
Category: Article
ISSN: 0265-9247
DOI: 10.1002/bies.20759

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Single‐cell microinjection has been successfully used to deliver exogenous proteins, cDNA constructs, peptides, drugs and particles into transfection‐challenged cells. With precisely controlled delivery dosage and timing, microinjection has been used in many studies of primary cultured cells, transgenic animal production, in vitro fertilization and RNA inference. This review discusses the advantages and limits of microinjection as a mechanical delivery method and its applications to attached and suspended cells. BioEssays 30:606–610, 2008. © 2008 Wiley Periodicals, Inc.

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