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Vitamin K-dependent γ-carboxyglutamic acid formation by kidney microsomes in vitro

✍ Scribed by Peter V. Hauschka; Paul A. Friedman; Hector P. Traverso; Paul M. Gallop


Book ID
118310873
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
423 KB
Volume
71
Category
Article
ISSN
0006-291X

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We report the first direct method for the identification of the vitamin K-dependent Ca2+ binding amino acid, gamma-carboxyglutamic acid (Gla), in the sequencing of proteins. The carboxyl groups on the protein are first converted to methyl esters with methanolic HCl, a procedure that reduces the pola