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Vitamin K dependent formation of γ-carboxyglutamate residues in tumor microsomes

✍ Scribed by Steven D. Buchthal; Cathy G. McAllister; David C. Laux; Robert G. Bell


Book ID
115755913
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
428 KB
Volume
109
Category
Article
ISSN
0006-291X

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We report the first direct method for the identification of the vitamin K-dependent Ca2+ binding amino acid, gamma-carboxyglutamic acid (Gla), in the sequencing of proteins. The carboxyl groups on the protein are first converted to methyl esters with methanolic HCl, a procedure that reduces the pola